ERPRINANDA GALUH BERLIANA, 061511133108 (2019) IDENTIFIKASI ISOLAT WNA 4.1.7 ASAL SEDIMEN MANGROVE WONOREJO SURABAYA DAN UJI AKTIVITAS BIOSURFAKTAN SERTA ANTIJAMUR Candida albicans ATCC 10231. Skripsi thesis, Universitas Airlangga.

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Abstract

The purpose of this researh was to identity the Genus of bacteria that produce biosurfactant which has antifungal activity to Candida albicans ATCC 10231. The bateria named WNA 4.1.7 isolated from Mangrove Wonorejo Surabaya. Mangrove Wonorejo Surabaya known contaminated of heavy metals. Heavy metals could induce microorganism to produce biosurfactant. Because of biosurfactant have amphiphilic characteristic that can reduce the surface tension, biosurfactant was for therapeutic and biomedical importance, such as antibacterial, antifungal and antiviral properties. Method Biosurfactant activity test used oil spreading, drops collapse, and parafilm test and antifungal activity method used diffusion agar. The result of this research showed that Genus of WNA 4.1.7 isolate was belong to Bacillus because WNA 4.1.7 isolate was Gram positive, bacil shape, aerob, and having oval endospore in center of the cell. The result of biosurfactant activity by oil spreading method showed by clear zone. The average clear zone was 49.55 mm, the average droplets of parafilm test was 6.63 mm, and the result drops collapse test was positive showed by spreading and drowned cell free supernatant of WNA 4.1.7. Cell free supernatant of WNA 4.1.7 could inhibit growth of Candida albicans ATCC 10231. The average inhibitory diameter is 28.45 mm. The conclusion of this research proved that WNA 4.1.7 isolate belong to Genus Bacillus which have biosurfactant and antifungal of Candida albicans.

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