LILA NARISYA, 021511133150 (2019) DAYA ANTIJAMUR EKSTRAK KITOSAN DARI BUBUK SQUID PEN TERHADAP Candida albicans. Skripsi thesis, Universitas Airlangga.

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Abstract

Chitosan is a natural material that can be obtained from fishery waste such as crustacean shells and squid pens obtained from squid. Squid pen is a waste produced from the processing of squid with transparent structures with feather-like shapes attached to the dorsal inside a squid mantle. Chitosan from squid pen has been developed in the medical field because it has biocompatible properties, biodegradable, non toxic, and also has antifungal activity. Being able to eliminate the Candida albicans fungi which is a predisposing factor for the occurrence of denture stomatitis due to the use of long-term dentures. Purpose: This study aims to determine the antifungal activity of chitosan extracted from squid pen against Candida albicans. Methods: Candida albicans was planted in Saboraud broth liquid media tubes and given chitosan suspension 1.5% (w/v) with each sample group consisting of 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0.78% and there are two control groups namely positive and negative controls. All samples were incubated for 24 hours at 37℃. Then a visual observation of turbidity on liquid Saboraud broth media was then carried out by planting culture on SDA media with 7 replications, incubated for 2x24 hours at 37 ℃ and counted the number of living colonies. The results were analyzed using Kruskall Wallis and Post hoc tukey test. Result: At the concentration of 25% there was no fungal growth in saboraud dextrose agar media. Conclusion: Chitosan that extracted from squid pens has antifungal activity against Candida albicans fungi with minimal concentration 25%.

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