Dwi Kusuma Wahyuni and Siti Nur Hafida and Tri Muji Ermayanti and Bambang Prajoga Eko Wardoyo and Hery Purnobasuki and Edy Setiti Wida Utami (2017) Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510. Biosaintifika Journal of Biology & Biology Education, 9 (1). pp. 26-32. ISSN 2085-191X
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Abstract
Gandarusa (Justicia gendarussa Burm.f.) is an Indonesian medicinal plant that has many benefits as drug and male contracetive. For industrial needs, Gandarusa must be available in large quantity. Hairy root culture is one of methode to produce phytochemistry compound. The objective of the study was to examine the effect of various density of Agrobacterium rhizogenes strain LB510 on hairy roots induction of gandarusa (Justicia gendarussa Burm.f.) leaf plant. Leaf explants were inoculated in MS liquid medium with various density of OD 600 = 0.1; 0.2; 0.3; 0.4; and 0.5. Explants were co-cultivated for 2 days on MS solid medium without any hormone then sub-cultured on MS solid medium containing antibiotic cefotaxim 300 ppm, in dark condition. The data were analyzed descriptively and statistically. The results showed that various density of Agrobacterium rhizogenes strain LB510 was affected the lenght of hairy roots induction of J. gendarussa Burm.f., but these was not effected toward lenght formation time and number of hairy root. The treatment of OD 600 0.2 was the best treatment for hairy root induction on Justicia gendarussa Burm. f. This data could be used for optimized the quality of methode of hairy root induction.
| Item Type: | Article | ||||||||||||||
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| Uncontrolled Keywords: | Agrobacterium rhizogenes; LB510 strain; density; hairy root culture; Justicia gendarussa Burm. f. | ||||||||||||||
| Subjects: | Q Science > Q Science (General) | ||||||||||||||
| Divisions: | 08. Fakultas Sains dan Teknologi > Biologi | ||||||||||||||
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| Depositing User: | Mr Akhmad Nurfizal Reza | ||||||||||||||
| Date Deposited: | 25 Jun 2019 07:41 | ||||||||||||||
| Last Modified: | 25 Jun 2019 07:41 | ||||||||||||||
| URI: | http://repository.unair.ac.id/id/eprint/83561 | ||||||||||||||
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Hairy Root Induction on Justicia gendarussa by Various Density of
Agrobacterium rhizogenes strain LB 510. (deposited 02 Mar 2022 02:26)
- Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510. (deposited 25 Jun 2019 07:41) [Currently Displayed]
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