HENY ASTUTIK, NIM011517017325 (2019) MEKANISME KONTRAKSI SEL MIOMETRIUM PADA MENCIT BUNTING SETELAH PEMBERIAN EKSTRAK RUMPUT FATIMAH (Anastatica hierochuntica L.) MELALUI EKSPRESI OTR, FP, Cx43, KADAR PGE2 DAN PGF2α. Disertasi thesis, Universitas Airlangga.

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Abstract

Objective: This study aimed to analyze the mechanism of myometrial contractions in pregnant mice after the administration of Rumput fatimah (Anastatica hierochuntica L.) extract through the expression of connexin-43, OTR and FP receptors and prostaglandin levels. Methods: The research was a randomized post-test only control group design. Twenty-one pregnant mice (Mus musculus) Balb/C strain which met the sampling criteria were divided into three groups, each of which consisted of 7 mice. They were classified as control group (P0) and two treated groups with administrations of Rumput fatimah (Anastatica hierochuntica L.) extract of different doses (P1 = 100 mg/kg BW; P2 = 150 mg/kg BW). Extracts were given per sonde for 5 days from the 14th to 18th day of pregnancy. Termination was carried out on the 19th day of pregnancy to take blood serum and uterine tissue for bioassay/ELISA, immunohistochemical staining and examination of uterine contractions using Powerlab. The results obtained were analyzed by employing comparative tests and path analysis. Results: There were significant differences in the increase in expression of connexin-43, OTR, and FP receptors in pregnant mice myometrium cell between treated groups and the control group (p <0.05), and there were significant differences in prostaglandin levels (PGE2, PGF2α ) between the control and the treated groups (p < 0.05). The administration of Rumput fatimah (Anastatica hierochuntica L.) extract increased the frequency of contractions (p = 0.014, p <0.05) and the amplitude of contraction (p = 0.017, p<0.05) but did not cause an increase in duration of contraction (p = 0.388, p > 0.05). The results of path analysis showed that rumput fatimah (Anastatica hierochuntica L.) extract increased contraction of pregnant mice myometrium cells through the OTR receptor pathway. Conclusion: Rumput fatimah (Anastatica hierochuntica L.) extract increases the expression of OTR, FP, Cx43, PGE2, and PGF2α levels and uterine contractions, therefore it is potential as a uterotropic agent.

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