Tamara Yuanita and Dania Vergeina Putri and Mandojo Rukmo and Nanik Zubaidah and Dian Agustin Wahjuningrum and Sri Kunarti (2017) Antibiofilm Power of Cocoa Bean Pod Husk Extract (Theobroma Cacao) Against Entercoccus Faecalis Bacteria (In Vitro). In: Proceeding of International Medical Device and Technology Conference (iMEDiTEC) 2017. Universitas Teknologi Malaysia, Johor Bahru. ISBN 978-967-0194-93-6

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Abstract

Endodontic pathogens which can form biofilms in the root canal. To remove E. faecalis biofilms, that must be taken is EPS degradation by oxidizing agents. Cacao bean pod husk (Theobroma cacao) is one of the potential herbal plants containing antimicrobial substances. The purpose of this study is to analyze the anti-biofilm activity of cacao bean pod husk extract against E. faecalis biofilms. Method: E. faecalis cells were cultured in microtiter plates. Cells remaining adhered to the wells were subsequently stained with crystal violet. After 24 hours of incubation, the optical density of each well was measured. Cocoa bean pod husk extract showed a decrease in the OD value at concentrations 100%, 50%, 25%, 12.5%, 6.25%, and 3.12% were lower than the OD values at concentrations of 1.56%, 0.78%, 0.39%, 0.19% as well as in the control groups. In conclusion, the Minimum Biofilm Inhibitory Concentration (MBIC) of cocoa bean pod husk extract is 3.12% concentration.

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