Bacillus licheniformis BT5.9 Isolated from Changar Hot Spring, Malang, Indonesia, as a Potential Producer of Thermostable α-amylase Repository

Bacillus licheniformis BT5.9 Isolated from Changar Hot Spring, Malang, Indonesia, as a Potential Producer of Thermostable α-amylase

Darah Ibrahim and Han Li Zhu and Nuraqilah Yusof and Isnaeni and Lim Sheh Hong (2013) Bacillus licheniformis BT5.9 Isolated from Changar Hot Spring, Malang, Indonesia, as a Potential Producer of Thermostable α-amylase. Tropical Life Sciences Research, 24 (1). pp. 71-84. ISSN 1985-3718

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Official URL: http://www.tlsr.usm.my/tlsr24012013/24012013_07.pd...

Abstract

A total of 34 bacterial isolates were obtained from soil samples collected from Changar Hot Spring, Malang, indonesia. Of these, 13 isolates produced a zone of hydrolysis in starch-nutrient agar medium and generated various amylases in liquid medium. One isolate was selected as the best amylase producer and was identified as Bacillus licheniformis BT5.9. The improvement of culture conditions (initial medium pH of 5.0, cultivation temperature of 50°C, agitation speed of 100 rpm and inoculum size of 1.7 × 109 cells/ml) provided the highest amylase production (0.327 U/ml).

Item Type:

Article

Uncontrolled Keywords:

Amylase, Thermophilic Bacteria, Bacillus licheniformis, Hot Spring

Subjects:

R Medicine
R Medicine > RS Pharmacy and materia medica

Divisions:

01. Fakultas Kedokteran > Ilmu Forensik

Creators: