Sholihatil Hidayati and Junaidi Khotib, NIDN. 0022107001 and Suharjono (2018) T-Cell Activation Controlling Effects of Ethyl Acetate Fraction of Kalanchoe Pinnata (LMK) Pes on TMPD-Treated Lupus Mice. International Journal of Pharmaceutical Sciences and Research, 9 (2). pp. 475-482. ISSN 2320-5148

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	Text (SIMILARITY) T-CELL ACTIVATION CONTROLLING EFFECTS OF ETHYL ACETATE FRACTION OF KALANCHOE PINNATA (LMK) PERS ON TMPD-TREATED LUPUS MICE.pdf Download (4MB)
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Abstract

Objective: The present study was designed to investigate the influence of sodium orthovanadate (SOV) on the P53 and caspase 3 expressions in beta cells of alloxan-induced diabetic mice. Methods: Babl/C strain mice induced diabetic condition with an intraperitoneal injection of 200 mg/kgBW alloxan monohydrate. After third days, alloxan-induced diabetic mice treated with SOV for 7 d. The pancreas was harvested on ten day and was stained using routine histology staining with hematoxylin-eosin (HE) and aldehyde fuchsin (AF) for morphological analysis and immunohistochemical approaches to observe the expressions of P53 and caspase 3 in the pancreas. Results: SOV reduced the fasting blood glucose levels after 7 d treatment at diabetic mice, respectively 303.0±126.8, 231.8±57.1 and 75.6±40.8 mg/dL. The results of histology staining of a pancreas showed that SOV reduced apoptosis in beta cells. Using immunohistochemical approaches, SOV might decrease the P53 and caspase 3 expressions in beta cells alloxan-induced diabetic mice. Conclusion: This study reveals that the administration of SOV on diabetic mice led to the decreasing of P53 and caspase 3 expressions that cause the decreasing of apoptosis in beta cells.

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