Hendy Hendarto, 090315218 D and Mohammad Fery Komarhadi and Erva Darmawanti and Widjiati and Suhatno and Fedik Abdul Rantam (2013) The Effect of Bone Marrow Transplantation on Oocyte Granulosa Cell Interaction and Follicular Development of Cisplatin-Induced Ovarian Failure in Rat. Journal of Stem Cell Research & Therapy, 3 (8). pp. 1-4. ISSN 2157-7633

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Abstract

Introduction: Chemotherapy has cytotoxic effect that induces follicular damage and abnormal folliculogenesisleads to ovarian failure. Two crucial growth factors in abnormal folliculogenesis, Growth Differentiation Factor-9 (GDF-9) and Kit-Ligand, will be disrupted and affect follicular development. In this study, we evaluate whether bone marrow transplantation (BMT) has a role on oocyte-granulosa cell interaction by analyzing GDF-9 and Kit-Ligand expressions and also follicular development by analyzing primordial, primary, secondary and graafian follicles of cisplatin-induced ovarian failure in rat. Material and method: Forty eight rats were divided into three groups: control, cisplatin and cisplatin+BMT. Ovarian failure was induced by administration of intraperitoneal cisplatin 5 mg/kg body weight for 1 week. BMT 2 × 107 cells were injected through rat tail vein after cisplatin administration. Bone marrow was isolated from rat femur and characterized by CD44(+), CD45(-), CD105(+). Immunohistochemistry examinations for ovarian GDF-9, Kit-Ligand and follicle development evaluation were performed after 2 weeks of BMT injection. Results: The expressions of Kit-Ligand among three groups by ANOVA were significant different (p=0.00), whereas by Post Hoc: cisplatin group lower than control group (p=0.00); cisplatin+BMT group higher than cisplatin group (p=0.00); and no significant different between control group and cisplatin+BMT group (p=0.955). The expressions of GDF-9 by Kruskal Wallis showed significant different (p=0.00) among three groups whereas cisplatin+BMT group higher than cisplatin group and control group. In cisplatin+BMT group the number of primordial, primary, secondary and graafian follicles were higher than those in cisplatin group; but were lower than those in control group (p=0.000). Positive Paul Karl Horan (PKH) labeling was seen in cisplatin+BMT group only.

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