Fifteen Aprila Fajrin, 050012273 and Junaidi Khotib, NIDN. 0022107001 and Imam Susilo, NIDN. 0010086503 (2013) HISTOLOGI DORSAL HORN DARI SPINAL CORD MENCIT YANG MENGALAMI NYERI INFLAMASI AKIBAT INDUKSI CFA (COMPLETED FREUD’S ADJUVANT) SETELAH PEMBERIAN GABAPENTIN DAN BACLOFEN. Buletin Penelitian Kesehatan, 41 (4). pp. 225-236. ISSN 0125-9695

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Abstract

Pain is a multidimentional experience, generally most of chronic disease followed by pain incidence. Chronic pain can be caused by inflammation or neuropathic condition and change the imbalance of N-methyl-D-aspartate receptor 2B (NR2B) subunit. This research was an observation of the influence of gabapentin and baclofen to dorsal horn histology in inflammatory-induced chronic pain. Fourty mice Balb-C strain were divided into 8 groups i.e sham, negative control, gabapentin at three different doses (10, 30, 100 nmol) and baclofen at three different doses (1, 10, 30 nmol). Inflammatory condition was induced by intraplantar injection of CFA (Completed Freud’s Adjuvant). Gabapentin and baclofen were given intrathecally once a day for seven consecutive days, at a week after CFA injection. Latency time toward thermal stimulus was measured on days 0, 1, 3, 5, 7, 8, 10, 12 and 14 after CFA injection. Paw thickness at the ipsilateral site was also measured on days 0, 1, 2, 3, 4, 5, 6, 7, 8, 10, 12 and 14 after CFA injection. Histology of the dorsal horn of spinal cord tissue was examined by haematoxylline-eosin staining. The result showed that intrathecal injection gabapentin and baclofen significantly increased latency time of mice toward thermal stimulus compared to negative control. Whereas gabapentin and baclofen administration could decrease inflammatory cell, vasodilatation and increase neuron forming of the dorsal horn histology compare to negative control. The conclusion of this research was gabapentin and baclofen administration had antinociceptic effect by increased latency time toward thermal stimulus and recoved histology of dorsal horn from mice with inflammatory pain

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