RYSKI MEIRINA WIENDANITA, NIM011428016307 (2019) PENGARUH PEMBERIAN NATRIUM DIKLOFENAK TERHADAP KONTRAKTILITAS SEL, DEPOSISI DAN DEGRADASI KOLAGEN LENSA PADA MODEL FIBROSIS KAPSUL LENSA ANTERIOR IN VITRO (Studi Eksperimental Laboratorium). Thesis thesis, Universitas Airlangga.

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Abstract

Purpose: to investigate the effect of natrium diclofenac on cell contractility, collagen synthesis and degradation in human lens epithelial cells (HLEC) of cataract congenital patient. Methods: HLEC were isolated from anterior lens capsule of cataract congenital patient. HLEC were divided into 4 groups consist of FBS 10% control group, natrium diclofenac 30 μg/ml, 100 μg/ml and 200 μg/ml treated group. This study investigated the effect of natrium diclofenac on cell contractility, collagen synthesis and degradation in HLEC. The collagen synthesis and degradation were determined by Sirius red binding assay, while cell contractility was analyzed by collagen contraction assay. The differences of cell contractility, collagen synthesis and degradation among groups were analyzed using Anova or Kruskal Wallis test followed by posthoc test with significant level of p<0.05. Results: Natrium diclofenac 30 μg/ml (19±6.15ug/mL) and natrium diclofenac 100 μg/ml (12.75±4.031ug/mL) significantly decreased collagen synthesis in line with induction of collagen degradation (1783±129.8ug/mL and 1793±1001ug/mL, respectively) in HLEC compared to FBS 10% control (collagen deposition 27.08±4.518ug/mL, p=0.000 and collagen degradation 1060±136.7ug/mL, p=0.000). Natrium diclofenac 30 μg/ml (13.055±4.61%), natrium diclofenac 100 μg/ml (13.39±3.20%), and natrium diclofenac 200 μg/ml (11.26±1,65%) were significantly decreased cell contractility compared to FBS 10% control group (40.918±6.67%, p=0.004). Conclusion: Natrium diclofenac might have antifibrotic effect on HLEC through extracellular matrix remodeling.

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