M, GANDUL ATIK YULIANI, 061217117302 (2019) KARAKTERISASI MOLEKULER DAN MAPPING EPITOP PROTEIN MEMBRAN LUAR Aeromonas hydrophilla ISOLAT LOKAL SEBAGAI KANDIDAT VAKSIN AEROMONIASIS. Disertasi thesis, Universitas Airlangga.

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Abstract

Aeromoniasis disease or known as Ulcer Diseases caused by Aeromonas hydrophila which in fish or shrimp often occurs in some embankment or in the Aquarium can cause sign patches – patches of bleeding can even cause economic loss and death if not promptly treated with treatment that accompanied the good sanitation. This disease affects many farmed freshwater fish in East Java. Previous research has managed to do the characterization of antigenic protein originating from the OMP (outer membrane protein) Aeromonas hydrophilla. Because it is felt necessary to do DNA sequensing encoder protein in question. To achieve these goals then do some exploratory research laboratoric methods, namely: identification of the bacterium Aeromonas hydrophilla fish from suspect infection by PCR, gel electrophoresis and Sequencing of gene fragments of the agarosa OMP A. hydrophilla isolates of East Java. The results obtained from this research is agarosa gel electrophoresis in DNA chromosome Aeromonas hydrophila bacteria cells is carried out at a temperature of annealing 54oC position 400 bp and the results of the sequencing primer that is used produces a sequence of nucleotides is around 355 nucleotides.

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