EKO WIDAYANTO, NIM011418016302 (2019) PENGARUH PEMBERIAN MEDIA TERKONDISI SEL PUNCA MESENKIMAL LIMBUS TERHADAP KETEBALAN KORNEA DAN EKSPRESI NA+/K+ ATPase SEL ENDOTEL KORNEA PASCA PAPARAN ULTRASOUND FAKOEMULSIFIKASI Penelitian Eksperimental Pada Oryctolagus Cuniculus. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Objective : to evaluate the effect of limbal mesenchymal stem cell (LMSC) conditioned media on corneal thickness (CCT) and Na+/K+ ATPase endothelial cells expression in rabbit eyes. Material and methods: This study was a true experimental laboratory study on rabbit pre and post test only control group design to evaluate the effect of giving limbal mesenchymal stem cell (LMSC) conditioned media on corneal thickness (CCT), and post test only control group design to evaluate the effect of administration LMSC conditioned media for the expression of Na + / K + ATPase corneal endothelial cells after exposure to phacoemulsification ultrasound. 24 New Zealand white rabbits (24 eyes) were divided randomly into 2 groups. Group A with right eye as control while group B with right eye as treatment. The control group was the group with the treatment of phacoemulsification which was given an injection of 0.2 ml of physiological saline (BSS) as a follow-up procedure while the treatment group was the group with injection of 0.2 SPML-conditioned media. Evaluation corneal thickness on 3rd days post phacoemulsification. Eyes were enucleated after 3 days to evaluate Na+/K+ ATPase Results: this study showed in the treatment group an increase in corneal thickness (50.00  36.45 micrometers, p = 0.001) with in the larger control group (62.00  27.41 micrometers, p = 0,000), but statistically there were no significant differences between the two groups (p = 0.372, ⍺ <0.05). Similarly, the observation of Na + / K + ATPase expression in the treatment and control groups from the results of observations of anatomical pathology of the histo-chemical coloring of antibodies did not show differences (p = 0.973, ⍺ <0.05). Conclusion: Effect of limbal mesenchymal stem cells (LMSC) conditioned media shows that on the third day after the phacoemulsification action there is still a failure of Na + / K + ATPase pump function in corneal endothelial cells and corneal edema occurs.

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