MELLISA DIAH PITALOKA, 050810121 (2012) VALIDASI METODE ANALISIS VITAMIN B1, B2, DAN B6 DALAM TABLET DENGAN KCKT MENGGUNAKAN KOLOM MONOLITIK RP-18 ENDCAPPED KEMURNIAN TINGGI. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

A simple reversed-phase HPLC method was developed and validated for the determination of Vitamin B1, B2 and B6 in tablet using high pure silica monolithic RP-18 end-capped column. The good separation was achieved with a mixture of methanol and water containing 6 mM hexanesulphonic acid, 0.2% trietanolamine (20:80) as mobile phase at flow rate of 1 ml/min, detected by Diode Array Detector at 270 nm. The method showed good linearity (r = 0.999) for all analytes with correlation coefficient of the function (V xo) were less than 2%. The accuracy of the method was in the range of accepted limits with average recovery of Thiamine, Riboflavin, and Pyridoxine was 100.38 ± 0.94 %; 100.51 ± 0.74 %; and 100.15 ± 0.74 %, respectively. The precision for Thiamine, Riboflavin, and Pyridoxine were 0.42 %; 0.31% and 0.18%, respectively. The proposed method can be applied for simultaneously determination of Thiamine, Riboflavin, and Pyridoxine in tablet

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