SITI NAFISAH, 051511133058 (2019) AKTIVITAS ANTIBAKTERI MIKROSFER PROBIOTIK Lactobacillus acidophilus FNCC- 0051 DENGAN MATRIKS NATRIUM ALGINAT TERHADAP Propionibacterium acnes ATCC 11827 PADA pH 4,5 DAN 6,0. Skripsi thesis, Universitas Airlangga.

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Abstract

The aim of this study is to analyze antibacterial activity of probiotic Lactobacillus acidophilus FNCC-0051 microsphere in buffer media pH 4,5 and 6,0. The microsphere using sodium alginate 2% as matrix and CaCl2 1,0 M as cross-linked solution. Antibacterial activity test completed by diffusion method using Propionibacterium acnes ATCC 11827 as a bacteria experiment. Antibacterial activity shown by inhibitory zone. The inhibitory zone was presented to a linear regression equation y = 6,6000x + 6,3191. The results had an antibacterial activity equivalence of probiotic microsphere to clindamycin phosphate against Propionibacterium acnes ATCC 11827. The results in pH 4,5 were 10,11 ± 1,07; 12,16 ± 1,25; 15,73 ± 2,36; 18,81 ± 3,20; 22,99 ± 2,38; 30,74 ± 5,24 ppm and the results in pH 6,0 were 20,98 ± 2,41; 25,58 ± 2,40; 31,04 ± 3,57; 36,09 ± 2,62; 40,47 ± 2,12; 48,38 ± 4,73 ppm. The antibacterial activity of probiotic microsphere in pH 6,0 is bigger than the antibacterial activity of probiotic microsphere in pH 4,5 (p<0,05).

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