Dhonna Mardiana and Budi Utomo and Mufasirin (2015) Pengaruh Pemberian Infusa Kulit Manggis (Garcinia mangostana L.) Terhadap Motilitas, Viabilitas dan Integritas Membran Spermatozoa Tikus Putih (Rattus norvegicus). Ovozoa, 4 (1). pp. 22-30. ISSN 2302-6464

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Abstract

The purpose of this research was to know the effect on mangosteen peel infuse for spermatozoa guality including the motility, viability and membrane integrity of spermatozoa. The method used for preparing the mangosteen peel infuse in this research was done by cutting the mangosteen peel in to small pieces then dried in the oven at 45”C, after that make it smoothed using a dry blender and finally boiled it at 90”C for 15 minutes. The giving administration of mangosteen peel infusion in mice was done orally as much as 2 ml appropriate with rat gastric content using 5 ml syringe attached with with canula. The consentration of mangosteen peel was 5 Yo, 10 Yo and 15 Yo and used vitamin C for the positive control also aguabides for the negative control. This research result was analyzed with ANOVA followed by Tukey test (p « 0.05). The result showed that there was no significant different level between the negative and positive control group with 5Y6 of mangosteen peel infusion level. There had very significant difference level for 10Yo and 15Yo of mangosteen peel infusion level and control group. So, the optimum consentration was 10Y6 and the maximum consentration was 15 Yo of the mangosteen peel infusion level. Key words: Spermatozoa, mangosteen peel infuse, motility, viability, membrane integrity

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