Thein Thein Myint and Muhammad Miftahussurur and Ratha Korn Vilaichone and New Ni and Than Than Aye and Phawinee Subsomwong and Tomohisa Uchida and Varocha Mahachai and Yoshio Yamaoka (2018) Characterizing helicobacter pylori cagA in Myanmar. Gut and Liver, 12 (1). pp. 51-57. ISSN 19762283

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Abstract

Background/Aims: Differences in the Helicobacter pylori infection rate are not sufficient to clarify the dissimilarity of gastric cancer incidence between Myanmar and its neighboring countries. To better understand this trend, the H. pylori virulence gene cagA was characterized in Myanmar. Methods: Glutamate-proline-isoleucine-Tyrosine-Alanine (EPIYA) patterns and CagA multimerization (CM) motifs of cagA genotypes were examined by performing polymerase chain reactions and DNA sequencing. Results: Of 69 tested H. pylori strains, cagA-positive patients had significantly more severe histological scores in their antrum than cagA-negative patients. Sequence analysis revealed that 94.1 of strains had Western-Type cagA containing an EPIYA motif (92.6) or EPIYT motif (6.4). The intestinal metaplasia scores in the antral of patients infected with the ABC and ABCC types of cagA were significantly higher than those of patients with AB-Type cagA. Interestingly, in patients infected with H. pylori, 46.3 of strains with three EPIYA motifs contained two identical Western-Typical CM motifs, and these patients showed significantly higher antrum inflammation scores than patients infected with two identical nontypical-CM motif strains (p=0.02). Conclusions: In Myanmarese strains, Western-Type cagA was predominant. The presence of CM motifs and the proportion of multiple EPIYA-C segments might partially explain the intermediate gastric cancer risk found in Myanmar.

Item Type:	Article
Additional Information:	(Review)
Uncontrolled Keywords:	bacterial DNA; CagA protein; bacterial antigen; bacterial protein; cagA protein, Helicobacter pylori, adult; Article; bacterial strain; bacterial virulence; cagA gene; cancer risk; controlled study; female; genetic analysis; genotype; Helicobacter pylori; human; human tissue; male; Myanmar; nonhuman; protein motif; protein multimerization; stomach cancer; 3' flanking region; DNA sequence; gastritis; genetics; Helicobacter infection; Helicobacter pylori; incidence; intestine; metaplasia; microbiology; middle aged; pathology; polymerase chain reaction; stomach antrum; stomach tumor, 3' Flanking Region; Adult; Antigens, Bacterial; Bacterial Proteins; Female; Gastritis; Helicobacter Infections; Helicobacter pylori; Humans; Incidence; Intestines; Male; Metaplasia; Middle Aged; Myanmar; Polymerase Chain Reaction; Pyloric Antrum; Sequence Analysis, DNA; Stomach Neoplasms
Subjects:	R Medicine > R Medicine (General) R Medicine > R Medicine (General) > R735-854 Medical education. Medical schools. Research
Divisions:	Artikel Ilmiah > SCOPUS INDEXED JOURNAL
Creators:	Creators NIM Thein Thein Myint UNSPECIFIED Muhammad Miftahussurur NIDN0029097909 Ratha Korn Vilaichone UNSPECIFIED New Ni UNSPECIFIED Than Than Aye UNSPECIFIED Phawinee Subsomwong UNSPECIFIED Tomohisa Uchida UNSPECIFIED Varocha Mahachai UNSPECIFIED Yoshio Yamaoka UNSPECIFIED
Contributors:	Contribution Name NIDN / NIDK Author UNSPECIFIED UNSPECIFIED Author UNSPECIFIED UNSPECIFIED Author UNSPECIFIED UNSPECIFIED Author UNSPECIFIED UNSPECIFIED Author UNSPECIFIED UNSPECIFIED Author UNSPECIFIED UNSPECIFIED Author UNSPECIFIED UNSPECIFIED Author UNSPECIFIED UNSPECIFIED Author UNSPECIFIED UNSPECIFIED
Depositing User:	PPJPI
Date Deposited:	12 Aug 2020 14:12
Last Modified:	12 Aug 2020 14:12
URI:	http://repository.unair.ac.id/id/eprint/94596
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