Ni Made Mertaniasih and Lindawati Alimsardjono and JF Palilingan and Soedarsono (2013) Rapid Culture Method Using Biphasic Media On Bacteriologic Diagnosis To Detect Mycobacterium tuberculosis For Determining Pulmonary Tuberculosis. Folia Medica Indonesiana, 49 (2). pp. 91-96. ISSN 0303-7932

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Abstract

Diagnosis to determine pulmonary TB based on detection of Mycobacterium tuberculosis rapidly and accurately is important todetermine the adequate therapy. Standard culture method using Lowenstein Jensen (L-J) media to detect and diagnoseMycobacterium tuberculosis require long time. Culture on solid clear medium may increase the sensitivity in examining the growthof mycobacterial colonies. Supplemented nutrition of media with sera in agar media and sera plus blood in broth phase media willincrease growth rate of colonies. Selectivity of culture media by added malachite green and penicillin. The objective was todetermine the validity of culture method using a biphasic blood-sera agar medium comparing with the standard culture method L-Jfor the detection of Mycobacterium tuberculosis from sputum specimens of 100 suspect pulmonary TB. The nutrition composition ofbiphasic media blood-sera agar which optimum colony growth of mycobacteria are 3.3gram nutrient agar (Oxoid) in 90ml aquadest,25μg malachite green, 3ml glycerol, 10ml sera, and 100U/ml penicillin, and broth phase contain similar nutrition by added blooddiluted; these biphasic culture method has a high laboratory sensitivity with limit detection of 102cfu/ml and high specificity forMycobacterium tuberculosis. Growth rate of mycobacterial colonies in biphasic media 10-14 days; biphasic method has high clinicalvalidity, 100% sensitivity and spesificity 100% to detect Mycobacterium tuberculosis in sputum of suspect pulmonary TB. Inconclusion, rapid culture method using biphasic media blood-sera agar for bacteriologic diagnosis to detect Mycobacteriumtuberculosis in sputum of suspect pulmonary TB patients have high sensitivity and specificity.

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