Ni Made Mertaniasih and Didik Handijatno and Agnes Dwi Sis Perwitasari and Desak Nyoman Surya Suameitria Dewi and Much Zaenal Fanani and Ika Qurrotul Afifah (2016) Sequence Analysis of the Gene Region Encoding ESAT-6, Ag85B, and Ag85 C Proteins from Clinical Isolates of Mycobacterium tuberculosis. In: Molecular and Cellular Life Sciences: Infectious Diseases, biochemistry and Structural Biology 2015 Conference, MCLS 2015. Procedia Chemistry, 18 . Elsevier, Surabaya, pp. 225-230. ISBN 9781510620708

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Abstract

Mycobacterium tuberculosis secreted proteins in culture filtrate and early phase of infection, such as early secretory antigen target 6 (ESAT6), culture filtrate protein 10 (CFP-10), and antigen 85 complex i.e. Ag85A, Ag85B, and Ag85C which played roles in adherence, invasion, cytolysis, and evading cytosol of macrophage, were virulence factors that determined the immune responses important on pathogenesis of Tuberculosis (TB), including granuloma formation or tissue that determine the degree of disease. The purpose of this research was to analyze the gene region sequence encoding ESAT-6, Ag85B, and Ag85C of Mycobacterium tuberculosis. Mycobacterium tuberculosis strain analyzed was taken from sputum of pulmonary TB patients in East Java, Indonesia. Sequenced DNA analyzed using GENETYX Ver.10. There were no SNPs both inside and outside epitope region of gene encoding ESAT-6, Ag85B, and Ag85C from clinical isolates of Mycobacterium tuberculosis. From this study, it could be concluded that the highly conserved gene region encoding ESAT-6, Ag85B, and Ag85C revealed no sequence polymorphism SNPs in epitope regions among Mycobacterium tuberculosis clinical isolates from sputum specimens of pulmonary TB patients.

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