Arif Rahman Nurdianto and Aryati and Mohammad Guritno Suryokusumo and Mufasirin (2019) Elevation of Bcl2 Expression in Spiralis Artery of Pregnant Rattus norvegicus Infected with tachyzoite of Toxoplasma gondii with Hyperbaric Oxygen Therapy. Qanun Medika Jurnal Kedokteran FK UM Surabaya, 3 (2). pp. 157-167. ISSN 2541-2272/e-ISSN 2548-9526

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Abstract

Spiral artery apoptosis plays a role in the process of abortion. Low Bcl2 expression found in abortion and in spiral artery. In pregnancy, Toxoplasmosis infection is found to increase apoptosis in spiral arteries. Hyperbaric Oxygen Therapy (HBOT) improves the expression of Bcl-2. This study aims to determine the effects of Hyperbaric Oxygen therapy in enhancing the expressions of Bcl2 in artery spiralis of pregnant rats infected with tachyzoite of Toxoplasma gondii. This is an experiment with a ‘randomized control group of post-test only design’ on 37 Rattus novergicus Sprague Dawley rats. Rats were divided into four groups. The group A is pregnant rats infected with 103 tachyzoites via intraperitoneal injection and received 10 sessions of HBOT 2.4 ATA in 3x30 minutes. Group B is pregnant only and received HBOT. Group C is pregnant and infected with tachyzoite but did not received HBOT. And the last, Group D is pregnant rats with no infection and did not received HBOT. Examinations of Bcl2 expressions were performed on day-5 after HBOT (twice a day). The Bcl2 expression was measured with immunohistochemistry. All data were tested with One-way ANOVA from SPSS 21. There is an increased expression of Bcl2 spiralis artery in the Group A. There was a significant difference between Group A and Group C with a value of p=0.042. HBOT can increase the expression of Bcl2 from the spiral arteries of rats, in the provision of HBOT 2.4 ATA for 3x30 minutes, 10 times in 5 days. Keywords:Toxoplasma gondii, tachyzoite, Hyperbaric Oxygen Therapy, Bcl2, spiralis artery

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