Aristika Dinaryanti, Aristika Dinaryanti and Deya Karsari, Deya Karsari and Nora Ertanti, Nora Ertanti and Igo Syaiful Ihsan, Igo Syaiful Ihsan and Aida Ariyanti, Aida Ariyanti and Fedik Abdul Rantam, Fedik Abdul Rantam and Aulani Aulanni’am, Aulani Aulanni’am and Purwati, Purwati (2019) Isolation and Characterization of Skin Derived Mesenchymal Stem Cell (SMSCs) from New Zealand Rabbit, Oryctolagus Cuniculus: A in Vitro Study. Biochemical and Cellular Archives, 19 (2). pp. 4797-4801. ISSN 0972-5075

	Text (ARTIKEL) 12. Isolation and Characterization of Skin Derived Mesenchymal Stem Cell (SMSCS).pdf Download (260kB)
	Text (SIMILARITY) 12. TURNITIN ISOLATION AND CHARACTERIZATION OF SKIN DERIVED MESENCHYMAL STEM CELL (SMSCs) FROM NEW ZEALAND RABBIT, ORYCTOLAGUS CUNICULUS _ A IN VITRO STUDY.pdf Download (1MB)
	Text (REVIEWER) 12. PEER_REVIEWER ISOLATION AND CHARACTERIZATION OF SKIN DERIVED MESENCHYMAL STEM CELL (SMSCs) FROM NEW ZEALAND RABBIT, ORYCTOLAGUS CUNICUL.pdf Download (3MB)
	Text (KESESUAIAN BIDANG ILMU) 13. Isolation and Characterization of Skin Derived Mesenchymal Stem.pdf Download (362kB)

Abstract

Mesenchymal stem cells (MSCs) exhibit high proliferation and self-renewal capabilities and are critical for tissue repair and regeneration. Furthermore, they can be isolated from a variety of tissues and have many potential applications in the clinical setting. In this study, Skin Derived Mesenchymal Stem Cells (SMSCs) were isolated from a New Zealand rabbit and analyzed for the skin sampling area, separation method, culture conditions, primary and passage culture times, and cell surface markers. The result shows that SMSCs have surface markers of mesenchymal stem cells by immunofluorescence of CD105, CD90, CD73, CD44, and CD200 and the CD105, CD90, and CD73 positive markers were also confirmed with Flow cytometry. Furthermore, negative markers of CD45 and CD34 did not show in immunofluorescence but CD45, CD34, and CD133 were confirmed with Flow cytometry.

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