Eduardus Bimo Aksono Herupradoto (2011) Inducible nitric oxide synthase (iNOS) expression in the bursa of fabricius of broilers infected with virulent gumboro virus and provides extract Annona squamosa (Swetsop). In: Proceeding International Seminar and 2nd Congsress of SEAVSA: Increasing Animal production through zoonoses and reproductive disorder handling, and the implementation of biotechnology. Central Publishing and Printing of Airlangga University, Surabaya, pp. 281-285. ISBN 978-602-8967-51-8

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Abstract

The bursa of fabricius serves as an important tissue in the process of gumboro virus (infectious bursal diseases/IBD) pathogenesis, since B cells of the bursa harbor the cytolytic phase of gumboro replication cycle. In the present study, inducible nitric oxide synthase (iNOS) associated with gumboro virus infection in the bursa of fabricius of broilers and provides extracts Annona squamosa (Sweetsop) as a leading immunostimulant were investigated. This research using the 40 individuals one-day-old broiler chickens (DOC). Broiler chicken experiment was obtained from livestock breeding. The cage was used multiple systems Cages measuring 20 x 15 x 10 em, each unit consisting of one head so that the number of cages all 40 units. Extraction of Annona squamosa (Sweetsop) is done at the laboratory of the Faculty of Pharmacy, Widya Mandala University. Annona squamosa (Sweetsop) used as test material was the result of reflux extraction methods using ethanol as solvent. The extract Annona squamosa (Sweetsop) with a dose of 10 mg/kg body weight, administered orally at the age of 7 days until the end of the experiment, namely the age of 35 days, given gumboro virus infection at the age of 20 days in a peroral dose of EID50 107• The design was as follows: Group A: given the extract of Annona squamosa (Sweetsop) without infected gumboro virus; Group B: without a given extract Annona squamosa (Sweetsop) and without infected gumboro virus; Group C: given the Annona squamosa (Sweetsop) and infected with gumboro virus; Group 0: without any extract Annona squamosa (Sweetsop) and given infected with gumboro virus. iNOS expression examination by immunohistochemistry method performed at the Laboratory of Faculty of Medicine, Airlangga University. The study began in June 2010 to October 2010. From these results prove that the expression of iNOS was higher in the bursa of gumboro virus infected broilers without any extract Annona squamosa (Sweetsop) when compared to uninfected controls and provides Annona squamosa (Sweetsop) (p<0.05). Keyword: iNOS, IBD, Annona squamosa, Bursa fabricius

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