Athi’ Purnasari (2020) Pengaruh Natrium Diklofenak Terhadap Enzim Matrix Metalloproteinase-3 Dan Kolagen Tipe I Pada Model Fibrosis Kapsul Anterior In Vitro (Studi Eksperimental). Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background: Posterior capsule opacification (PCO) is one of the problems that arise in pediatric cataracts. Natrium diclofenac, a non-steroidal anti-inflammatory drug, at certain doses have shown inhibition of proliferation of lens epithelial cell (LEC) cultures. Methods: Human LEC recruited from anterior lens capsule of congenital cataract patients. The scratching technique on LEC cultures made in-vitro PCO model. HLEC culture were divided into 4 groups consist of FBS 10% control group, natrium diclofenac 30 μg/ml, 100 μg/ml, and μg/ml group. The expression of matrix metalloproteinase-3 (MMP-3) and type I collagen between groups were analyzed. The data were analyzed using Mann-Whitney and Anova test then followed by Posthoc test with significant level of p<0.05. Results: Natrium diclofenac 30 μg/ml (69.12 ± 6.87 pixels), natrium diclofenac 100 μg/ml (73.42 ± 9.23 pixels) and natrium diclofenac 200 μg/ml (74.20 ± 6.45 pixels) increase MMP-3 expression significantly compared with the control (54.79 ± 6.04 pixels, p=0.00). Natrium diclofenac 30 μg/ml, 100 μg/ml and 200 μg/ml significantly decreased type I collagen (47.15 ± 7.19 pixels, 38.12 ± 5.25 pixels, 42.56 ± 6.46 pixels, respectively) compared with the control (1.01 x 102 pixels, p<0.05). Conclusion: Natrium diclofenac has fibrosis inhibitory effect on LEC through MMP-3 and type I collagen

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