AUILIARAHMA RIDHA, 050911035 (2013) PENETAPAN KADAR KOLKISIN DALAM SEDIAAN SIRUP CAIR DAUN KEMBANG SUNGSANG (Gloriosa superba L.) DENGAN METODE KLT - DENSITOMETRI. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Quantitative analysis related with the determination of how much of a certain substance contained in a sample. In an analysis, a series of steps must be followed for the purpose of analysis by using a specific technique called the method of analysis. Validation method performed to ensure that an analytical method meets the criteria and requirements of good method of analysis. Liquid syrup of Gloriosa superba L. leaf is a new inovasion product and there was no method validation applied before. The aim of this study was determining colchicine in liquid syrup of G. superba L. leaf. Validation is done first by setting the parameter category I, accuracy, precision, specificity, linearity and range. Analysis method was using TLC-Densitometry with Silica gel 60 F254 layers and a mixture of chloroform:diethylamin (8:2) as mobile phase. The result shows coefficient of correlation (r) were 0,9975 (r table was 0,917 for n = 6 and α = 0,01) with the linear regression y =17,8521x+10014,14; Vxo = 2,68%; and range 2,55. The recovery was 94,06 ± 8,53 % with coefficient of variation of 1,44%. Application of this method for determination colchicine from liquid syrup of G. superba L. leaf showed that average content of colchicine is 0,4847 gram/ 10 ml. As conclusion, this method was valid for validation of colchicine in sample.

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