KINASIH AICHA PUTRI, 051011147 (2013) OPTIMASI KONDISI DAN VALIDASI METODE ANALISIS ACID VIOLET 43 DALAM PEWARNA RAMBUT SECARA KROMATOGRAFI CAIR KINERJA TINGGI. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

HPLC method was optimized and validated for analysis of acid violet 43 in hair dye. Separation was performed on a column Purospher® STAR RP-18 endcapped using methanol: 0.1% formic acid (80:20) as the mobile phase with a flow rate of 1 mL/min and wavelength was set at 254 nm. The method gave a good chromatographic peak and separately with other compounds with Resolution (Rs) greater than 1.5. Validation study indicated that the proposed method showed good linearity (r = 0.999) over the range of 10-30 ppm with a correlation coefficient of the function (Vxo) of less than 2%. Accuracy and precision of the method was in the range of accepted limits with average recovery of acid violet 43 was 98.54 ± 2.12 % and RSD below 5.3%. Method detection limits for acid violet 43 was 0.57 ppm.

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