Poedji Hastutiek and Agus Sunarso and R Heru Prasetyo (2017) Permot (Passiflora foetida Linn.) leaf extracts as bioinsecticide againts in relation Aedes aegypti larvae. The Southest Asian Journal of Tropical Medicine and Public Health, 48 (6). pp. 1169-1174. ISSN 0125-1562

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Abstract

The aim of this study was to investigate the effectiveness of Passiflora foetida Linn. extracted by various organic solvents (n-hexane, ethyl acetate and ethanol) against IV instar Aedes aegypti larvae. The components fractionated by thin layer chromatography of n-hexane extract were alkaloids and terpenoids, that of ethyl acetate fraction was terpenoid, and that of ethanol fraction was phenol. Gas chromatography-mass spectrometry analysis of n-hexane fraction indicated that the presence of isophytol, neophytadiene, 9,12,15-octadecatrienoic acid, 13-octadecenal, and phytol; and of the n-hexane fraction as isophytol and phytol. Larvae (n = 25) were exposed to each organic solvent extract (0, 500, 1,000, 1,500, and 2,000 mg/l) for 24 hours, each experiment conducted four times. Mortality data were analyzed using probit analysis at 95% significance level. The 50% lethal concentration of n-hexane extract, 440 mg/l, was the lowest, but too high to be used effectively against Aedes aegypti larvae. Further purification will be required to identify the active larvicidal compound(s). Keywords: Aedes aegypti, Passiflora foetida, fourth instar larva, larvicidal activity

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