Nazaratun Thaiyibah and Amalia Rozaiza Ightikhoma and Ahmad Yudianto (2018) The Effect of Seawater on The Quantity of Dental Pulped DNA in Forensic Odontology Identification. In: Proceedings of the 2nd International Conference Postgraduate School (ICPS 2018). Science and Technology Publications, Lda., Surabaya, pp. 440-445. ISBN 978-989-758-348-3

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Abstract

The identification of forensic odontology on victims of sea natural disasters is highly efficient. Its contrast with the identification of soft tissue that are susceptible to damage is a consequence of seawater exposure. Seawater affects the decomposition of corpses and creates potential difference due to salinity (content of salt) in the water. The identification of odontology can consist of DNA analysis of the dental pulp, because the dental pulp is shielded by hard tissue such as dentine and enamel which are makes the pulp capable to protect the DNA, but the enamel is semipermeable (water permeable), which can affect the extraction of DNA degree and its purity. This study aims to determine the effectiveness of seawater to the quantity of teeth pulp DNA. The research was conducted experimentally by observing and explaining situations that occurred (cause and effect) in dental pulp DNA quantity exposure to seawater from 1 day and 7 days using spectrophotometer method with wavelengths of 260 nm and 280 nm. The results of this study indicated that the waters of the Lombok Strait with 28.74 ‰ of salinity degraded the DNA content of the dental pulp, but the DNA purity remained stable.

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