Sinta Purwaningtyas (2020) Efek Parameter Proses Freeze Drying Terhadap Biokompatibilitas Kolagen-Hidroksiapatit Yang Didoping ZNO. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Damage to bones can occur due to several things such as defects, accidents, lack of minerals in the bones due to diet, even the effects of unfavorable activities. Efforts made in dealing with damage to the bone required biomaterials as bone substitutes. The ingredients used in making scaffold are hydroxyapatite and collagen which are doped with ZnO using the freeze drying process. This review article aims to determine the effect of time and temperature variations of the hydroxyapatite composite on the freeze drying method on biocompatibility characteristics for bone scaffold applications. The method used to find out which scaffold is good for the body by conducting a review of 10 international journals. Review 10 of this journal include several characterization tests to determine physical properties, namely the FTIR test, SEM test, and porosity test. In addition, biocompatibility tests are performed through the MTT Assay test. Variations in temperature and freezing time result in an increase in pores in the hydroxyapatite-collagen composite scaffold. Increased pore size can affect cell viability. Based on the results of a review of 10 journals, hydroxyapatite-collagen composite scaffold produced a large pore diameter size of ± 200-500 μm, porosity of ± 50%, and the percentage of living cells above 100%, while the addition of alginate to hydroxyapatite resulted in a pore size of ± 200-500 μm and the percentage of living cells is 70-100%, and the addition of chitosan to hydroxyapatite produces a pore size of 30-129%, porosity of 40-80%, and the percentage of living cells above 90-120%. The addition of ZnO to hydroxyapatite composites results in a decrease in pore size and increased porosity because small zinc ions can occupy small volumes Aminatun, M.Husni, Jan Ady, Dyah H.2019. Synthesis and Characterization of Nano-Hidroxyapatite/Chitosan/Carboxymethyl Cellulose Composite Scaffold. 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