Dewi Nur Sholikhah, - and Sri Pantja Madyawati, - and Wiwiek Tyasningsih, - and Lianny Nangoi, - and Pudji Srianto, - (2017) Non Specific Bacteria Isolate in Reproductive Tract of Dairy Cattle That Experienced Repeat Breeder at KSU "Tunas Setia Baru" Pasuruan Regency. International Journal of Development Research, 7 (9). pp. 15598-15601. ISSN 2230-9926

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Abstract

The study aims to isolate genus of non specific bacteria in reproductive tract of dairy cattle that experienced Repeat Breeder at KSU Tunas Setia Baru, Pasuruan Regency.. Bacteria isolate was done using Gram coloring, Catalase test, Motility test, Spore test, further bio-chemical test using medium of TSIA, Mannitol and Glucose. Result of observation on 10 bacteria samples was Gram positive bacteria of coccus with white round colony and those that changed MSA medium to be yellowish 2/10 (20%) were samples of number 4 and 5. Gram negative bacteria of coccobacil with methalic green round colony on EMBA medium 5/10 (50%) was obtained from samples number 2,4,7,9 and 10. Gram positive bacteria of bacil and white round colony on medium of TSA and BA 5/10 (50 %) was obtained from samples number 1, 3, 6, 8 and 9. The study concluded that non specific bacteria that was able to be isolated was those from Corynebacterium genus 4/10 (40%), Staphylococcus genus 2/10 (20%) and Escherichia genus 5/10 (50%) Keywords: Isolate, Non Specific Bacteria, Dairy Cattle, Repeat Breeder Case

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