Christian Khoswanto, - (2020) A High Sucrose Diet Affects Calcium Levels and the Number of Osteoblasts in the Wistar Rat Extraction Socket. Journal of International Dental and Medical Research, 13 (1). pp. 134-137. ISSN 1309-100X

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Abstract

The important role from wound regeneration is the availability of sufficient calcium minerals for the process of mineralization in the bone after tooth extraction. Bone in the mandibulocraniofacial complex is a process of intramembranous ossification, which originally originated from mesenchymal connective tissue. Several studies have shown the role of a diet high in sucrose capable of causing bone loss, promoting osteoclast differentiation and bone resorption. A high diet of sucrose is also able to increase calcium levels in plasma, increasing calcium levels in urine. The purpose of this research is to know the effect of a high sucrose diet affects calcium levels and the number of osteoblasts in the Wistar rat extraction socket. This study is an experimental laboratory research using The Post-Test Only Control Group Design. 40 male Wistar Rats weigh between 150-200 grams, 3 months of age are being used. Have well condition, food and drink water given ad libitum. The 40 rats are divided into four groups. The data were analyzed statistically using One-Way ANOVA and LSD0.05. Result: The result at 30 day examined via a light beam that is transmitted through the tissue using image magnified 400 times shows a significant decrease (p<0.05) in amount number of osteoblast and bone calcium level when the experimental groups (T3) were compared with the control group (C), T1 and T2. High sucrose diet decreasing the number of osteoblasts and alveolar bone calcium contents in the Wistar rat extraction socket.

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