Indra Mulyawan, - and Andra Rizqiawan, - and Pratiwi Soesilowati, - and David Buntoro Kamadjaja, - (2021) Expression of TNF- and MMP-13 Following Subcutaneous Implantation of Demineralized Freeze Dried Bovine Cortical Bone Membrane in Rat’s Dorsum. Journal of International Dental and Medical Research, 14 (1). pp. 74-78. ISSN 1309-100X

	Text (NASKAH) Expression of TNF-and MMP-13 Following Subcitaneous Implantation of Demineralized Freeze.pdf Download (549kB)
	Text (TURNITIN) 18. Expression of TNF- and MMP-13 Following Subcutaneous Implantation of Demineralized Freeze Dried Bovine Cortical Bone Membrane in Rat’s Dorsum.pdf Download (2MB)
	Text (VALIDASI) 18.Dr.Pratiwi (Expression of TNF-and MMP....).pdf Download (942kB)

Abstract

Guided Bone Regeneration (GBR) for alveolar bone augmentation requires combination of bone graft and tissue barrier in the form of biodegradable membrane. Bovine pericardium collagen membrane (BPCM) is the most commonly used resorbable membrane. Demineralized freeze dried bovine cortical bone membrane (DFDBCBM) was known to undergo faster biodegradation compared to BPCM. The study attempts to reveal the mechanism of DFDBCBM degradation by analyzing Tumor Necrosis Factor-Alpha (TNF-) and Matrix Metalloproteinase – 13 (MMP-13) expression after implantation of DFDBCBM and BPCM in rat’s dorsum. This experimental study used 60 male rat’s which are divided into 2 groups. Subcutaneous implantation of DFDBCBM were done in the first group, while BPCM implanted in the control group. On day 2, 5, 7, 14, 21 and 28 post implantation five rats were sacrificed from each group for immunohistochemistry staining to analyze TNF- and MMP-13 expression. Comparative and correlation study were done to analyze the two parameters. There was no significant differences in TNF- and MMP-13 expression between the two groups over all observation periods. However, no correlation was found between TNF- and MMP-13 expression in both groups.

Actions (login required)

View Item