Antimicrobial Activity of Musa paradisiaca var. sapientum on Enterococcus faecalis Viability

Hendrik Setia Budi, - and Wisnu Setyari Juliastuti, - and Brenda Regina Christy, - (2020) Antimicrobial Activity of Musa paradisiaca var. sapientum on Enterococcus faecalis Viability. Malaysian Journal of Medicine and Health Sciences, - (-). pp. 17-21. ISSN 2636-9346

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Official URL: https://medic.upm.edu.my/upload/dokumen/2020070611...

Abstract

Introduction: Root canal treatment often fails because of bacteria that grow back after obturation, such as Entero¬coccus faecalis (E. faecalis). The previous study has found the percentage of root canal treatment failure as much as 32-70%. It was because the facultative anaerobic bacteria are able to penetrate into the dentinal tubules and found to be resistant. Ambonese banana stem is one of the herbal plants in Indonesia that has been widely used, including the sap on the stem. Active substances in the sap of Ambonese banana stems such as flavonoids, saponins, and tannins have antimicrobial efficacy. The purpose of this study is to prove the antibacterial activity of Ambonese banana stems extract on E. faecalis and analyzing bacterial viability through a fluorescent microscope. Methods: The sample of this study was E. faecalis (ATCC 29212) which bred on BHIB media, then serially carried out dilution with Ambonese banana stem extract with a composition of 100%, 50%, 25%, 12.5%, 6.25%, 3.125 %, 1,563%. and 0.781%. Cul¬tured in the Agar Mueller-Hinton medium and then calculate the number of bacterial colonies. This examination was continued using a fluorescent microscope for determining the viability of bacteria. Results: We obtained MIC at a concentration of 1.563% and MBC at a concentration of 3.125%. The ability of Ambonese banana stem extracts to kill E. faecalis in MIC was 92.22%, while in MBC it was 100%. Conclusion: Ambonese banana stem extract effec¬tively inhibited the growth of E. faecalis bacteria.

Item Type: Article
Uncontrolled Keywords: Ambonese banana stem extract, Enterococcus faecalis, MIC, MBC, Fluorescent microscope
Subjects: R Medicine
R Medicine > RK Dentistry > RK1-715 Dentistry
Divisions: 02. Fakultas Kedokteran Gigi > Oral Biology
Creators:
CreatorsNIM
Hendrik Setia Budi, -NIDN0010067204
Wisnu Setyari Juliastuti, --
Brenda Regina Christy, --
Depositing User: Muhammad Fadli Rois
Date Deposited: 04 Jul 2022 06:24
Last Modified: 20 Apr 2023 04:43
URI: http://repository.unair.ac.id/id/eprint/116934
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