Anis Satus Syarifah, - and Sri Agus Sudjarwo, - and Hendy Hendarto, - and Reny I'tishom, - (2020) Nanocurcumin Protective Effect on Lipid Peroxide of Lead Acetate Induced White Rats (Rattus norvegicus) Ovarian Granulosa Cells. Indian Journal Forensic Medicine & Toxicology, 14 (4). pp. 764-769. ISSN 0973-9122

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Abstract

Objective: This study aims to investigate the protective effect of nanocurcumin on lipid peroxidation of rat ovarian granulosa cells induced by lead acetate (Pb). Methods : A total of 45 mice were divided into a negative control group (C-) receiving corn oil, a positive control group (C+) receiving corn oil and lead acetate (Pb) 40 mg/kg bw/day, experimental group 1 (E1), experimental group 2 (E2), experimental group 3 (E3) receiving 50, 100 and 200 mg/kg/day nanocurcumin dissolved in corn oil and lead acetate (Pb) 40 mg/kg bw/day. The treatment was given orally and the treatment time was daily at 08.00 AM for 26 days. Ovaries were removed for immunohistochemical analysis of MDA expression. Result : The positive control group had the highest MDA expression of ovarian granulosa cells (94.2 ± 10.5) and was significantly different (p <0.05) from negative control group (1.5 ± 1.4), experimental group 1 (17.4 ± 10.5), experimental group 2 (11.4 ± 8.5) and experimental group 3 (7.2 ± 4.0). Conclusion : Nanocurcumin as a protective oxidative stress on ovarian granulosa cells due to exposure to lead acetate, by protecting lipid peroxidation.

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