Jola Rahmahani, - and Maha Kirana, - and Fedik Abdul Rantam, - and Suwarno, - (2022) Peneguhan Diagnosis Penyakit Inclusion Bodies Hepatitis pada Ayam Broiler Menggunakan Metode Polymerase Chain Reaction. Jurnal Medik Veteriner, 5 (2). pp. 145-148. ISSN 2615-7497, E ISSN 2581-012X

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Abstract

This study aimed to diagnose cases of Inclusion Bodies Hepatitis (IBH) in broiler chickens. A total of 6 chickens were collected from farms in Banten and Jember. Samples were taken from the liver which underwent changes such as brittle, yellowish in color and enlarged. The samples were ground and extracted, then the Polymerase Chain Reaction (PCR) test was performed using Primers Hexon A (5'-CAARTTCAGRCAGACGGT-3') and Hexon B ( 5'-TAGTGATGMCGSGACATCAT-3' ) with 897 bp length. The results showed that the broiler chicken case sample from Banten was positively 100% infected by the IBH Virus. Keywords : Inclusion Bodies Hepatitis, Polymerase Chain Reaction, broiler

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