Erma Safitri, - and R. Heru Prasetyo, - and Mas'ud Hariadi, - and Pudji Srianto, - (2018) Hypoxic Precondition for Induce Pluripotency of Rabbit’s Bone Marrow-Derived Mesenchymal Stem Cells. Biochemical and Cellular Archives, 18 (2). pp. 1509-1517. ISSN 0972-5075 e-ISSN 0976-1772

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Abstract

Hypoxic precondition in vitro culture can be induced of pluripotency from MSCs like hypoxia niche 1-3% in rabbit’s bone marrow reside. This study was done in two phases: First phase, hypoxia precondition treatment on the stem cells culture with several O2 concentration (21, 1 and 3%) and several cultivation time (1, 2, 4, 8 days); Second phase, analysis of pluripotency based on genotype expression of OCT4 and SOX2 genes after hypoxia precondition treatment. The analysis of pluripotency: expression of genotype OCT4 and SOX2 genes from the third groups sample, revealed cDNA chain at 114 bp (OCT4) and 319 bp (SOX2). But based on analyses in the same line, only 2 groups sample was found result gene sequenz of OCT4 and SOX2 code similarity with whole genom that found in gene bank was on sample that cultured with hypoxia (1% and 3% O2). The matching of OCT4 gene sequenz with the data based on similarity significance value cut off that must > 60% (73% in culture 1% O2 and 65% in culture 3%O2), eventhough in normoxia culture (21%O2) similarity significance value cut off < 60% (only 43%). The matching of SOX2 gene sequenz with the data based on similarity significance value cut-off > 60% (85% in cultur 1% O2 and 3% O2), eventhough in normoxia culture (21% O) similarity significance value cut off <60% (only 50%). Conclusions of this study : Hypoxic precondition (1% and 3% O2) and cultivation time (2, 4, 8 days) in vitro culture can be induced of pluripotency from rabbit’s MSCs based on genotype expression of OCT4 and SOX2 genes with similarity significance value cut off that must ≥ 60%.

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