Masami Kanawa, - and Akira Igarashi, - and Katsumi Fujimoto, - and Tania Saskianti, - and Ayumu Nakashima, - and Yukihito Hi- 4 gashi, - and Hidemi Kurihara, - and Yukio Kato, - and Takeshi Kawamoto, - (2021) The Identification of Marker Genes for Predicting the Osteogenic Differentiation Potential of Mesenchymal Stromal Cells. Current Issue Molecular Biology. ISSN 14673037, 14673045

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Abstract

Mesenchymal stromal cells (MSCs) have the potential to differentiate into a variety of ma- 22 ture cell types and are a promising source of regenerative medicine. The success of regenerative 23 medicine using MSCs strongly depends on their differentiation potential. In this study, we sought 24 to identify marker genes for predicting the osteogenic differentiation potential by comparing ilium 25 MSC and fibroblast samples. We measured the mRNA levels of 95 candidate genes in nine ilium 26 MSC and four fibroblast samples before osteogenic induction, and compared them with alkaline 27 phosphatase (ALP) activity as a marker of osteogenic differentiation after induction. We identified 28 17 genes whose mRNA expression levels positively correlated with ALP activity. Next, we com- 29 pared the mRNA levels of the 17 genes in ilium MSCs with those in jaw MSCs, which have different 30 differentiation properties. The levels of seven out of the 17 genes were not substantially different 31 between jaw and ilium MSCs, while the remaining 10 genes were expressed at significantly lower 32 levels in jaw MSCs than in ilium MSCs. The mRNA levels of the seven similarly expressed genes 33 were also compared with those in fibroblasts, which have little or no osteogenic differentiation po- 34 tential. Among the seven genes, the mRNA levels of IGF1 and SRGN in all MSCs examined were 35 higher than those in any of the fibroblasts. These results suggest that measuring the mRNA levels 36 of IGF1 and SRGN before osteogenic induction will provide useful information for selecting com- 37 petent MSCs for regenerative medicine.

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