The effects of laserpunctureongonadalmaturityandspermqualityof male stripedcatfish (Pangasianodonhypophthalmus)

Akhmad Taufiq Mukti, ,- and Y.G.P.Sari, ,- and G.S.R.Agusdinata, ,- and W.H.Satyantini, ,- and A.S.Mubarak, ,- and E.M. Luqman, ,- and Widjiati, ,- (2020) The effects of laserpunctureongonadalmaturityandspermqualityof male stripedcatfish (Pangasianodonhypophthalmus). Theriogenology, 147 (-). pp. 102-107. ISSN -

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Official URL: https://www.sciencedirect.com/science/article/pii/...

Abstract

Laserpunctureisoneoftheapplicativetechnologiesusedmainlyinanimaland fish reproductions. Laserpuncturetechnologyhasbeenusedtoimprovegonadalmaturityandspermqualityin fish rapidly. This studyaimedtodeterminetheeffectsofdifferentlaserpuncturedosesongonadalmaturityand sperm qualityofmalestripedcatfish. Malesstripedcatfish (800e900g/fish bodyweight)andIgonadal maturitystagewereused.Semi-conductorsoftlaserwasusedwithdosesof0.2-,0.4-,and0.5-J,while the negativecontrol(withoutthelaserpunctureandtheovaprim™) andonlytheovaprim™ wereused as acomparisontreatment,respectively.Thesoft-laserwastreatedonreproductiveacupointeveryweek for fourweeks,whiletheovaprim™ wasadministeredbyintramuscularinjectionatdoseof0.2mL/kg fish in final rearingperiod.Fishwasrearedinhapaatthecontrolledpond.Fishwasfedwithacom- mercial feedcontaining32%crudeprotein.Gonadalmaturity,gonadosomaticindex(GSI),hepatosomatic index(HSI),andspermqualityofmalestripedcatfish weremeasuredinthe final rearingperiod.The results showedthatthelaserpunctureonthereproductiveacupointhadahighlysignificanteffect (P < 0.01)onthegonadalmaturity,GSI,HSI,andspermqualityofmalestripedcatfish. Intermsofthe gonadal maturity,laserpuncturedosestreatmentof0.4and0.5-JgavethemostmatureIVstage.While the highestlevelsofGSIandHSIwerefoundin0.5-Joflaserpuncturedose,whichwas2.17%and1.54%, respectively.Inaddition,thebestspermqualitieswereobservedin0.5-Joflaserpuncturedose,which were81.75%motility,82.75%viability,and7.0 � 109 cell/mLconcentration.Theseresultssuggestthatthe laserpuncturecanaccelerateagonadalmaturityandimprovespermqualityinmalestripedcatfish.

Item Type: Article
Uncontrolled Keywords: Laserpuncture Semi-conductorsoftlaser Gonadal maturity Sperm quality Male stripedcatfish
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
S Agriculture > SH Aquaculture. Fisheries. Angling > SH1-691 Aquaculture. Fisheries. Angling > SH20.3-191 Aquaculture
Divisions: 14. Fakultas Perikanan dan Kelautan > Akuakultur
Creators:
CreatorsNIM
Akhmad Taufiq Mukti, ,-NIDN0008037404
Y.G.P.Sari, ,-UNSPECIFIED
G.S.R.Agusdinata, ,-UNSPECIFIED
W.H.Satyantini, ,-UNSPECIFIED
A.S.Mubarak, ,-UNSPECIFIED
E.M. Luqman, ,-UNSPECIFIED
Widjiati, ,-UNSPECIFIED
Depositing User: ghishella ayu rahmawati
Date Deposited: 28 Apr 2023 03:22
Last Modified: 28 Apr 2023 03:22
URI: http://repository.unair.ac.id/id/eprint/123522
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