Dewi Nurmalita Suseno, ,- and Epy Muhammad Luqman, ,- and Mirni Lamid, ,- and Akhmad Taufiq Mukti, ,- and Muhammad Agus Suprayudi, ,- (2020) Residual impact of 17@methyltestosterone and histopathological changes in sexreversed Nile tilapia (Oreochromis niloticus). Asian Pacific Journal of Reproduction, 9 (1). pp. 37-43. ISSN 2305-0500

	Text (Artikel) C1.2.pdf Download (4MB)
	Text (Plagiasi) C1.2_compressed_new.pdf Download (1MB)
	Text (Korespondensi) C1.2_compressed_new.pdf Download (3MB)
	Text (Penilaian Karil dan Kesesuaian Bidang Ilmu) C1.1-32 Karil Jurnal Ilmiah-2.pdf Download (927kB)
	Text (Keterangan Etik) 2-1. SK No. 959-2.pdf Download (186kB)

Abstract

To examine sex reversal both by oral and by immersion using 17毩-methyltestosterone on the methyltestosterone residual concentration and the organ histopathology of tilapia fish. Methods: This study used oral and immersion treatment methods for sex reversal of tilapia fish and used normal fish as the control and each treatment was repeated 4 times. 17毩-methyltestosterone at dosages of 60 mg/kg feed and 0.5 mg/L were used for oral and immersion methods, respectively. In the first step, tilapia fry were reared at 100 L aquaria, with a density of 1 fish/L for 2 months. In the next step, male tilapias were reared at happa (net cage) of (2×1×1) m3 size in the controlled pond, with a density of 30 fish/happa for 3 months. The methyltestosterone residual concentrations were analyzed by one-way analysis of variance and Duncan’s multiple range tests, while organ histopathology was analyzed by descriptive method. Results: Residual concentrations in the serum of methyltestosteronetreated fish were significantly lower than that in normal fish, especially in 4- and 5-month-old tilapias with averages of less than 5 μg/L, while in normal fish was more than 5 μg/L. In the flesh, methyltestosterone residual concentrations showed relatively no significant differences between the oral and immersion treatment groups and methyltestosterone-treated fish remained lower compared to normal fish, except in 5-month-old tilapia. Methyltestosterone-treated tilapia exhibited histopathological changes on gill, liver, kidneys, and intestine organs. Conclusions: Sex reversal either by oral or by immersion has methyltestosterone residual concentration, but does not exceed the limits (5 μg/L or 5 μg/kg) of synthetic steroid on the fish body, although methyltestosterone causes histopathological changes on gill, liver, kidneys, and intestine.

Actions (login required)

View Item