ALFIANA LAILI DWI AGUSTIN, 061314253009 (2016) IDENTIFIKASI MORFOLOGI DAN PROFIL PROTEIN NEMATODA Tanqua tiara STADIUM DEWASA YANG DITEMUKAN PADA LAMBUNG Varanus salvator. Thesis thesis, UNIVERSITAS AIRLANGGA.
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Abstract
Predilection of nematode Tanqua tiara (T. tiara) in the Varanus salvator (V. salvator) stomach, worm T. tiara very strongly attached to the abdominal wall and penetrate to the sub mucosa. hole formed in the abdominal wall Varanus, this formation occurs as a result of penetration of cephalic bulb performed by the worm, this penetration results in the epithelium of stomach damage and penetrate deep into the submucosa. Living environment of V. salvator in which there are around water such as swamps, drains / sewers and ponds led to worms found in V. salvator easily infect intermediate hosts and likely to infect mammals that drink water that has been contaminated by worm eggs T. tiara out with feces, although there are no reports of human infection T.tiara but all species have the potential to transmit disease to humans through direct contact or indirectly. This study was conducted to determine the molecular weight of the protein nematode T. tiara isolated from the stomach tract V. salvator using SDS-PAGE techniques and to analyze characterization protein antigenic T. tiara using western blot techniques. The method is carried out by sparation homogenates Whole Worm Extract (WWE) using techniques sodium doedecyl sulfate polyacrilamide gel electrophoresis (SDS-PAGE) followed by protein blotting with serum of experimental animals that have been infected WWE T. tiara. The results of SDS-PAGE protein bands in the form of 9 respectively 115, 99, 90, 83, 65, 54, 46, 37 and 30 kDa. While the western blot obtained 3 protein bands, 115, 46, and 30kDa.
| Item Type: | Thesis (Thesis) | |||||||||
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| Additional Information: | KKC KK TPKMV.03/16 Agu i | |||||||||
| Uncontrolled Keywords: | Morphology, Tanqua tiara, Varanus salvator, SDS-PAGE, Western blot. | |||||||||
| Subjects: | S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF780.2-780.7 Veterinary microbiology, bacteriology, virology, mycology | |||||||||
| Divisions: | 06. Fakultas Kedokteran Hewan > Ilmu Penyakit dan Kesehatan Masyarakat Veteriner | |||||||||
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| Depositing User: | Guruh Haris Raputra, S.Sos., M.M. '- | |||||||||
| Date Deposited: | 18 Apr 2016 06:53 | |||||||||
| Last Modified: | 18 Apr 2016 06:53 | |||||||||
| URI: | http://repository.unair.ac.id/id/eprint/29483 | |||||||||
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