Achmad Fuad Hafid, - and Ni Putu Ariantari, - and Lidya Tumewu, - and Agriana Rosmalina Hidayati, - and Aty Widyawaruyanti, - (2010) Determination of Marker Compound of Artocarpus champeden Spreng. Stembark Extract And Validation of The Analysis Method Using HPLC. In: International Conference On Medicinal Plants The Future of Medicinal Plant: From Plant to Medicine Surabaya, 21-22 July 2010, Faculty of Pharmacy Widya Mandala Chatolic University.

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Abstract

Artocarpus clnnpedel SPRENG. (Moraceae), commonly known as been traditionally used for malarial remedies. Several studies also reported in vitro and in vivo antimalarial activity of this plant. In order to devcloped A. champedetr srembark as anrimalarial phytopharmaceutical product, marker approach was chosen for quality control purpose of the exhact as mw material. The aims of this study are to determine marker compound of A. clompeden stembark extract and develop an analysis method for quantification of marker compound in ethanol extract of A. champeden SPRENG. stcmbark using HPLC. Isolation of marker isolate was caried out by column chromatography and prepantive TLc rechniques. Based on fIV, IR, 'H NMR, "c NMR, 2D NMR (CosY, luf) 2O1O "cempedak", HMBC and HMQC) and MS spectra and comparison with other refcr:nces, confirmed that marker isolatc was identical with known prcnylatcd chalcone, Morachalconc A. Thc HPLC profile showed that marker compound has time retcntion shown as Mean t SD (RSD) of 12.547 minutes 1 0.39 (3.08%). Validation of analysis method for quantification of marker in ethanol extract was conducted. The analyte were chromatographed on C8 250x4.6 mm column, methanol-water as mobile phase (65:35 v/v) by isocratic elution at flow rate of I m[-/minute, column temperature of 30'C, stop time of analysis at 20 minutes and detection wavclength at 385 nm. The method was validated in terms of selectivity, spccificity, linearity, accuracy and precision. The calibration curvc was linear within a high range concentration of 25 - 50 pgm.L-l and low range conccnuation of 3-5 - X pgml--r. The accuracy and intraday precision wcrc found to be well within acccptable limits. The marker content in ethanol extract ofA. champeden SPRENG. stembark was shown as M?all I SD (RSD) of 0.33617o I 0.03 (8.45%).

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