DIAH PURWANINGSARI, 091170121 (2018) POLYSACCHARIDE KRESTIN DARI CORIOLUS VERSICOLOR SEBAGAI IMUNOMODULATOR PADA PERBAIKAN ARTRITIS AJUVAN SENDI KAKI TIKUS (RATTUS NORVEGICUS). Disertasi thesis, Universitas Airlangga.

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Abstract

Polysaccharide krestin (PSK) is a protein-bound polysaccharide K obtained from the extraction process of C. versicolor fungus that has immunomodulatory properties. RA is one of the chronic inflammatory autoimmune diseases. The factors that are responsible for RA disease is a balance between the proinflammatory factor and an anti-inflammation factor ie. Th17, Regulatory T cell, Th1 and Th2. TGF-β1 acts as an anti-inflammatory cytokine in inducing the formation of Treg FoxP3 cells in rat and humans and can suppress effector T cells in autoimmune disease.The aim of this study is to explain the potency of PSK from C. versicolor as an immunomodulator against the improvement of Adjuvant arthritis in rat’s (Rattus norvegicus) joint feet. The research method used was experimental laboratory using a Randomized Post Test Only Group Design. The subjects were 66 male adult rats (Rattus norvegicus), aged 16 weeks with 200-250 grams BW, which had been induced by adjuvants (AA). Subjects were divided into 6 groups, 3 treatment groups and 3 control groups. PSK is given at a dose of 50 mg / kg BW / day through gastric sonde for 1, 2 and 3 weeks in 3 different groups. Elisa and Flowcitometry blood tests were performed to see levels of TGF-β1 and T regulator, joint tissue retrieval for histopathology and immunohistochemistry to see joint histopathology scale and MMP3 expression, as well as measurements on the foot thickness.. The results showed a significant difference in TGF-β1 1 week (p=0,017), 2 weeks (p<0,001) and 3 weeks (p<0,001), T Regulatory cell 1 week (p=0,008), 2 weeks (p<0,001) and 3 weeks (p<0,001), MMP3 expression 1, 2 and 3 weeks (p<0,001), histopathological joint scale 1, 2 and 3 weeks (p<0,001), foot thickness before sacrifice 1, 2 and 3 weeks (p<0,001) of PSK given. The correlation between PSK - TGF-β1 levels - CD4+CD25+FoxP3+ Regulatory T cell - MMP3 expression - histopathological joint scale - foot thickness during the second and third weeks showed a significant correlation with moderate to strong strength correlation. Conclusions: Provision of PSK as immunomodulator at dose 50 mg/ kg BW/ day orally potentially improved the Adjuvant Arthritis in the rat’s (Rattus norvegicus) foot joints through mechanism of increased TGF-β1 levels and CD4+CD25+FoxP3+ Regulatory T cells, decreased MMP3 expression, histopathological joint scale and rat’s foot thickness with the most effective given time of 2 weeks.

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