Ibrahim Njoto and Ayly Soekanto and Ernawati and Abdurachman, NIDN. 0011096602 and Handono Kalim and Kusworini Handono and Djoko W. Soeatmadji and Fatchiyah (2018) Chondrocyte Intracellular Matrix Strain Fields of Articular Cartilage Surface in Hyperglycemia Model of Rat: Cellular Morphological Study. Medical Archives : Journal of The Academy of Medical Science in Bosnia and Herzegovina, 72 (5). pp. 348-351. ISSN 0305-199x

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Abstract

Introduction: Chondrocyte is one cell in articular cartilage was products many proteins, molecules, and other factors. The external infl uence of cartilage, such as: hyperglycemia was entering joint capsule and impact to the chondrocytes and the cartilage. Hyperglycemia caused modifi cation of heparan sulfate proteoglycan 2 (perlecan) proteins through glycation process. Aim: The aim of this study was to analyze morphological changing of chondrocytes pericellular matrix by the infl uence of hyperglycemia. Material and Methods: Eighteen adult male rats were divided into six groups: control, rat treated with sugar intake was 0.5 mg/kg, 0.75 mg/kg, 1mg/kg, 1.5 g/kg and 2 mg/kg of body weight. The animal model was dislocated and left knee was taken to observe changing of chondrocytes pericellular matrix strain fi elds by Scanning Electron Microscope (SEM) from perpendicular to femoral condylus cartilage. Results: Changing of chondrocytes intracellular matrix strain fi elds as changing of cell diameters and cell distances at group control and group I to group V, which cell diameters was lower level and cell distances was the highest level at over diet 2. This changing of intracellular matrix strain fields was corresponding to changing chondrocytes morphology in hyperglycemia condition, due to hypertrophic stage as adaptive responses. This research as based on next research for accomplish of hyperglycemia infl uence to morphology articular cartilage changing to prevent degeneration of cartilage towards osteoarthritis. Conclusions: Present study concludes that hyperglycemia infl uence to chondrocyte intracellular matrix strain fi elds changing.

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