Tutik Sri Wahyuni and Adita Ayu permatasari and Tri Widiandani and Achmad Fuad Hafid and Aty Widyawaruyanti and Chie Aoki Utsubo and Hak Hotta (2018) Antiviral activities of curcuma genus against hepatitis C virus. Natural Product Communications, 13 (12). pp. 1579-1582. ISSN 1934-578X

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Abstract

Hepatitis C virus (HCV) infection is one of the major public health problems in the world. Even though the new agents are shown to increase the sustained virology response, however, there are still many people who cannot access the therapy due to the high cost. Moreover, the emergence of resistance and side effects presented the necessity to develop alternative treatment agents for HCV infection. Plants of the genus of curcuma are popular among traditional medicines in the world, including Indonesia. They have been used for many herb remedies and reported to possess many biological activities. Several plants from the curcuma genus were known as treatment agents in liver disease and jaundice. Our current study determines antiviral activities of Curcuma domestica, Curcuma xanthorrhiza, and Curcuma heyneana against HCV and further examines the mechanism of actions. Antiviral activity was performed by in vitro culture cells using Huh 7.5it cells and treated with the mixture of extract and virus JFH1. The effects of extracts in HCV life cycle were determined by mode of action analysis to examine the action of substances in the entry or post entry steps. The results revealed that ethanol extract of C. domestica, C. xanthorrhiza, and C. heyneana showed strong anti-HCV activities with IC50 values of 1.68 ± 0.05, 4.93 ± 0.42 and 5.49 ± 0.59 µg/mL, respectively without any cytotoxicity effect. Mode of action analysis demonstrated that of C. domestica and C. heyneana exhibit HCV in the entry step, while C. xanthorrhiza inhibit in the entry and post entry steps of HCV life cycle. Docking analysis to predict the interaction of curcumin, the main compound of curcuma genus, revealed a strong interaction between curcumin and 4GAG receptor, a protein involved in the entry step of HCV infection. Moreover, it was also reported to possess good interaction with 4EAW, an HCV NS5B, which plays an important role in HCV replication. These results suggested that C. domestica, C. xanthorrhiza, and C. Heyneana possessed strong inhibition against hepatitis C virus, therefore they may be good candidates for anti-HCV agents.

Item Type:	Article
Uncontrolled Keywords:	Hepatitis C virus, Curcuma domestica, Curcuma xanthorrhiza, Curcuma heyneana, Curcumin, Docking analysis.
Subjects:	R Medicine > RS Pharmacy and materia medica R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica R Medicine > RS Pharmacy and materia medica > RS200-201 Pharmaceutical dosage forms
Divisions:	05. Fakultas Farmasi
Creators:	Creators NIM Tutik Sri Wahyuni NIDN0025107704 Adita Ayu permatasari UNSPECIFIED Tri Widiandani NIDN0004128001 Achmad Fuad Hafid NIDN0012125207 Aty Widyawaruyanti NIDN0026046210 Chie Aoki Utsubo UNSPECIFIED Hak Hotta UNSPECIFIED
Depositing User:	Mr M. Fuad Sofyan
Date Deposited:	06 Jul 2019 05:42
Last Modified:	24 Jan 2020 02:09
URI:	http://repository.unair.ac.id/id/eprint/84663
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