ZAHRATUS SILMI ALIYAH, 051511133131 (2019) OPTIMASI SUMBER NITROGEN PADA PRODUKSI METABOLIT ANTIBAKTERI DARI Bacillus tequilensis BSM-F SIMBIOSIS Halichondria panicea ASAL PERAIRAN CABBIYA MADURA. Skripsi thesis, Universitas Airlangga.

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Abstract

hed because it can produce antibacterial metabolite, including Bacillus tequilensis BSM-F symbiosis Halichondria panicea from Cabbiya Madura Seawater. Bacillus tequilensis BSM-F in producing antibacterial metabolites is influenced by several factors, one of which is media. The purpose of this study was to determine the type and concentration of optimum nitrogen sources as a medium for the production of Bacillus tequilensis BSM-F symbiosis of Halichondria panicea. The method used in the production of Bacillus tequilensis BSM-F is solid fermentation, while the antibacterial activity test against Staphylococcus aureus ATCC 25923 and Eschericia coli ATCC 25922, which respectively represent Gram positive bacteria and Gram negative bacteria using agar diffusion method. Based on the results of the research conducted, the nitrogen source that can produce the optimum antibacterial metabolite is casein with a concentration of 1.5%.

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