Budi Utomo, NIDN. 0018055904 (2018) Identification Polymorphism of FSH ẞ-Sub Unit Gene and FSHR Gene in Madura Cattle Results of Artificial Insemination with Limousin Cattle with PCR-RFLP Technique. International Journal of Advanced Scientific Research and Management, 3 (10). pp. 38-43. ISSN 2455-6378

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Abstract

The aim of this study was estimate of polymorphisms located in the FSH β sub unite and the receptor Follicle Stimulating Hormone (FSHR). Fourteen blood samples were collected from Madura cattle results of Artificial Insemination with Limousin Cattle called “Madrasin” cattle which lived in Bangkalan, Madura Island. The DNA was extracted following standard methods. The purified DNA was subjected to PCR-RFLP techniques to identify polymorphisms of the FSH β sub unite and the receptor Follicle Stimulating Hormone (FSHR) genes in “Madrasin” cattle ecotypes. The Polymerase Chain Reaction – Restriction Fragment Lenght Polymorphism (PCR- RFLP) amplified fragments of FSH β sub unite (313 bp) were disgested with restriction enzymes PstI resulting in 255 bp, 237 bp and 150 bp fragments, that produced 2 allelic is A and B. The allelic A and B frequencies for FSH β sub unite of “Madrasin” cattle is 0,50 and 0,50. While, The Polymerase Chain Reaction – Restriction Fragment Lenght Polymorphism (PCR-RFLP) amplified fragments of FSHR (306 bp) were disgested with restriction enzymes AluI resulting become two homozigote genotypes, there are GG in the 243 bp, CC in the 243 bp and 193 bp. So the conclusion of this research was that FSH and FSHR genes not changed. Key words: Polymorphisym, MAS, FSH β-sub unite, FSHR, PCR-RFLP

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