Characterization and Production of Polyclonal Antibody Anti Excretory Secretory Protein of Blastocystis sp.

Briantono Willy Rendragraha, - and Lucia Tri Suwanti, - and Rahaju Ernawati, - and Mufasirin, - and Setiawan Koesdarto, - and Wiwiek Tyasningsih, - and Soelih Estoe Pangestie, - and Heni Puspitasari, - and Septian Hakim Susantoputro, - (2019) Characterization and Production of Polyclonal Antibody Anti Excretory Secretory Protein of Blastocystis sp. The Indian Veterinary Journal, 96 (12). pp. 30-32. ISSN 0019-6479, e-ISSN: 0974-9365

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Official URL: https://ivj.org.in/en/webhome.aspx

Abstract

This study aims to produce and characterize polyclonal antibody anti excretory secretory (ES) protein of Blastocystis sp. ES protein pro le was analyzed using SDS-PAGE and used to immunize 2 rabbits. Rabbit’s serum were analyzed using indirect ELISA and Western Blot. The result showed that molecular weight of ES protein of Blastocystis sp was 40 and 50 kDa and the protein was immunogenic. Both ES protein and antibody anti ES of Blastocystis sp can be promoted as diagnostic kit. Key words : Blastocystis sp, Excretory Secretory Protein, Polyclonal Antibody

Item Type: Article
Uncontrolled Keywords: Blastocystis sp, Excretory Secretory Protein, Polyclonal Antibody
Subjects: S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine
S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF811-909 Veterinary medicine of special organs, regions, and systems
Divisions: 06. Fakultas Kedokteran Hewan > Parasitologi Veteriner
Creators:
CreatorsNIM
Briantono Willy Rendragraha, --
Lucia Tri Suwanti, -NIDN0028086208
Rahaju Ernawati, -NIDN0003105002
Mufasirin, -NIDN0011076705
Setiawan Koesdarto, -NIDN0028095203
Wiwiek Tyasningsih, -NIDN0028036207
Soelih Estoe Pangestie, -NIDN0015095601
Heni Puspitasari, --
Septian Hakim Susantoputro, --
Depositing User: Nn Erna Dwi Indriyani
Date Deposited: 15 Apr 2020 03:26
Last Modified: 30 Jun 2022 00:29
URI: http://repository.unair.ac.id/id/eprint/95099
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