Wahyu Dewi Tamayanti, - and Ferawati, - and Iwan Sahrial Hamid, - and Elisabeth Widjaja Kusuma, - (2015) Mixture of Sambiloto (Andrographis panniculata Nees) and Salam (Syzygium polyanthum (Wight). Walp) Extract to Improve GLUT4 and PPAR-y Expression in Hyperglicemic Wistar Rats. In: International Seminar "Natural Resources Biotechnology: From Local to Global", 8-9 September 2015, Faculty of Biotechnology Universitas Atma Jaya Yogyakarta.

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Abstract

The increased prevalence of diabetes mellitus stimulates the ongoing study of molecular mechanisms of sambiloto (Andrographis panniculata Nees.) and salam (Syzygium polyanthum (Wight) Walp.) in relieving the disorder. The molecular study was done by treating hyperglicemia Wistar rats with a combination of sambiloto (AP) and salam (SP) extract. After 14 days of treatment, the rats were sacrificed and the liver organs were collected. The organs were stored in a solution of 10% buffered formalin to be blocked in paraffin afterwards. Subsequent molecular test was performed to identify GLUT4 and PPARY gene expression by immunohistochemistry method. This study showed that administration of a combination of sambiloto and salam extracts (6:1) in rats was able to increase the expression level of GLUT4 which resembled the expression level of GLUT4 after insulin administration. It was also found that the expression level of PPARy was increased after rats were treated by combination of AP and SP extracts (6:1). It showed that the increased expression of PPARY resembles the expression of PPARY after metformin stimulation. Interestingly, the administration of AP:SP extract (6:1) combination showed a level of GLUT4 expression that was not in line with PPARY expression.

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