Erma Safitri, - and Suzanita Utama, - and Thomas Valentinus Widiyatno, - (2014) Marrow Mesenchymal Stem Cell (MMSCs) Transplantation for the Improvement of Reproductive Function in Rat Testis Degeneration. Proceeding of The 3nd International Conference on Multidisciplinary Research (ICMR) 2014 Islamic University of North Sumatra. pp. 49-54. ISSN 978-602-18851-9-2

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Abstract

The purpose of this research was to obtain adaptive MMSCs for the therapy of testicular degeneration in rat. Adaptive MMSCs can be achieved by hypoxia precondition during in vitro culture. Hypoxia precondition in this research was conducted by applying 3% O2 which was then compared with those cultured in normoxic 21% O2. The specific target of this research was devided into two experiments, Experiment I was the isolation and identification of mesenchymal stem cell from rat bone marrow. Identification was confirmed by the expression of CD 105' and CD45 cells. In Experiment 2 cellular MMSCs testiculomyoplasty were performed on male rats that have been preconditioned with testicle degeneration. Next, the success was identified in vivo based on the expression of spermatogonial stem cell (SSCs) and the improvement of testicle tissue. Transplantation results showed that under 3% O2 concentration SSCs were expressed and testis tissue improvement occured. Meanwhile at 21% O2 concentration testis tissue improvement did not occur. The conclusion of this research showed that hypoxia precondition using 3% 0. concentration very support MMSCs to remain adaptive after being transplanted to patient with spermatogenesis impairment, in addition to the testicle tissue improvement.

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