TIFFANY TANDIAN, - (2022) Uji Aktivitas Antikanker Senyawa Pinostrobin Propionat terhadap Sel Kanker Payudara T47D dan Selektivitasnya terhadap Sel Normal Vero secara In Vitro. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Breast cancer is one of the most common malignant diseases and is the leading cause of death in women. So far, breast cancer treatment has not been effective as it affects normal cells. It is necessary to develop a new cancer drug that selectively kills cancer cells. This study aims to determine the anticancer activity of pinostrobin propionate compound as pinostrobin derivative against T47D breast cancer cell line and its selectivity against Vero normal cell line in vitro. Cytotoxicity test by MTT assay on T47D cells showed an IC50 value. The selectivity assay performed by the same method on Vero normal cells showed a CC50 value. Thus, the selectivity index (SI) can be obtained by calculating the ratio CC50 over IC50. In the cytotoxic assay, T47D cells were given pinostrobin concentrations of 100-2000 ?M and 50-1500 ?M for pinostrobin propionate. In the selectivity assay, Vero cells were given both compounds with concentrations of 50-1500 ?M. The results showed that pinostrobin propionate had a lower IC50 value (566 ?M) than pinostrobin (2932 ?M). Therefore, pinostrobin propionate had better cytotoxic activity against T47D cells. The results of the selectivity assay showed that pinostrobin propionate had a CC50 value of 938 ?M and pinostrobin had a CC50 value of 1271 ?M. The selectivity index of pinostrobin propionate is 2 which is more selective than pinostrobin. Therefore, pinostrobin propionate is a potential anticancer treatment for breast cancer in vitro which is selective in killing cancer cells.

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