Muhammad Luthfi, NIDN. 0006036704 and Retno Indrawati, NIDN. 0012115903 and Ira Arundina, NIDN. 0028107102 and Yoes Prijatna Dachlan (2015) Analyze Activation Marker of Azurophilic Granule (CD63) and CFSE CD11C Expressions of Salivary Neutrophils Insevere Early Chilhood Caries. In: Proceeding Molecular and Cellular Life Sciences: Infectious Diseases, Biochemistry and Structural Biology. Institute of Tropical Disease Universitas Airlangga, Surabaya. ISBN 978-602-14292-4-2

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Abstract

Early childhood cariesis is a very serious health problem because it is a chronic infectious disease that is infectious. In recent years the view has changed dramatically neutrophils, where neutrophils which are a key component of the first line of defense against pathogen through the process of phagocytosis. Neutrophils also release their granule contents such as defensins, Defensins (α defensins) are stored in the azurophilic granules have microbicidal function shown in enable macrophages to release tumor necrosis factor (TNF) and interferon γ (IFNγ) which promote pro inflammatory. α Defensin also increases the permeability of the epithelial monolayer in vitro and chemotactic effects on T cells, mast cells and dendritic cells. Purpose this research is to analyze activation marker of azhurophilic granules and CFSE CD11c expression of salivary neutrophils in severe early childhood caries (S-ECC). Two groups ie, results mouthwash NaCl 1.5% samples of 20 early childhood caries-free and 20 severe early childhood caries. Salivary neutrophils that collected from severe early childhood caries analyzed using flow cytometry to detect the CD63 and CFSE CD11C expressions. Based on the average value is known that salivary neutrophils expressing CD63+ in early childhood caries-free higher (2.67%  0.46) in comparison to the severe early childhood caries (2.32% 0.57), likewise salivary neutrophils expressing CFSECD11c+ in early childhood caries-free higher (2.44% ± 0.52) in comparison to the severe early childhood caries (1.57 ± 0.39). Decreased activation marker azhurophilic granules (CD63) and phagocytosis markers (CFSE CD11c) expressions may be one cause in the S-ECC.

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