Widya Paramita Lokapirnasari and Adriana Monica Sahidu and Tri Nurhajati and Koesnoto Supranianondo and Andreas Berny Yulianto (2016) Lactococcus lactis Lactic Acid Bacteria from Intestine Beef Cattle as a Candidate Probiotics. In: Proceeding International Seminar on Molecular Biology in Veterinary Medicine. Faculty of Veterinary Medicine Universitas Airlangga, Surabaya, pp. 62-64. ISBN 9786027043817

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Abstract

Probiotics is one feeds additive that can be used to increase production of animal husbandry and to reduce the use of antibiotic growth promoter (AGP). This research aims to identify isolates of lactic acid bacteria from intestine beef cattle that can be used as a candidate probiotics. Survival test on acidity was performed with some modification survival test on bile salts, antagonistic test on enteric pathogen was performed with agar diffusion method with modification in the pouring of pathogenic bacteria. The research showed that Lactococcus lactis showed viability in pH 2 as much as 6.9 x 10' CFU/ml and isolate viability on bile salts 3.9 x 10” CFU/ml. Antagonist test on Staphylococcus aureus showed inhibition diameter as much as 3.0 mm and in antagonist test on Escherichia coli as much as 3.5 mm. Based on the result of the research showed that Lactococcus lactis derived from intestine beef cattle has tolerant on acidity, bile salt and antagonistic on enteric pathogen £.coli dan S.aureus so it has potency as candidate probiotic. Key words : Lactococcus lactis, lactic acid bacteria, beef cattle intestine.

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